[ASAP] High-Titer Production of Olivetolic Acid and Analogs in Engineered Fungal Host Using a Nonplant Biosynthetic Pathway

Posted on Aug 19th, 2021 - By Ikechukwu C. Okorafor, Mengbin Chen, and Yi Tang

[ASAP] Characterizing and Improving Reaction Times for <italic toggle=”yes”>E. coli-</italic>Based Cell-Free Protein Synthesis

[ASAP] Metabolic Engineering of <italic toggle=”yes”>Escherichia coli</italic> for High-Yield Production of (<italic toggle=”yes”>R</italic>)-1,3-Butanediol

Posted on Jul 14th, 2021 - By Yu Liu, Xuecong Cen, Dehua Liu, and Zhen Chen

[ASAP] Formation of Giant Lipid Vesicle Containing Dual Functions Facilitates Outer Membrane Phospholipase

Posted on Jul 13th, 2021 - By Seren Ohnishi and Koki Kamiya

[ASAP] A Computational Metabolic Model for Engineered Production of Resveratrol in <italic toggle=”yes”>Escherichia coli</italic>

Posted on Jul 7th, 2021 - By Michael Cotner, Jixun Zhan, and Zhen Zhang

[ASAP] Development of a Suite of Tools for Genome Editing in <italic toggle=”yes”>Parageobacillus thermoglucosidasius</italic> and Their Use to Identify the Potential of a Native Plasmid in the Generation of Stable Engineered Strains

[ASAP] The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in <italic toggle=”yes”>E. coli</italic>

[ASAP] A Biosensor for Detection of Indole Metabolites

Posted on Jun 24th, 2021 - By Jiefei Wang, Chao Zhang, and W. Seth Childers

[ASAP] High-Yielding Terpene-Based Biofuel Production in <italic toggle=”yes”>Rhodobacter capsulatus</italic>

[ASAP] Production and Characterization of Motile and Chemotactic Bacterial Minicells

Posted on Jun 2nd, 2021 - By Bin Ni, Remy Colin, and Victor Sourjik

[ASAP] Functional Comparison of Laboratory-Evolved XNA Polymerases for Synthetic Biology

[ASAP] Accurate Detection of Rare Mutant Alleles by Target Base-Specific Cleavage with the CRISPR/Cas9 System

Posted on May 18th, 2021 - By Dongin Lee, Ji Hyun Lee, and Duhee Bang

[ASAP] Patterning Microtubule Network Organization Reshapes Cell-Like Compartments

[ASAP] Engineered Microbial Routes for Human Milk Oligosaccharides Synthesis

[ASAP] Transcription-Factor-based Biosensor Engineering for Applications in Synthetic Biology

Posted on Apr 24th, 2021 - By Nana Ding, Shenghu Zhou, and Yu Deng

[ASAP] The Tryptophan-Induced <italic toggle=”yes”>tnaC</italic> Ribosome Stalling Sequence Exposes High Amino Acid Cross-Talk That Can Be Mitigated by Removal of NusB for Higher Orthogonality

[ASAP] <italic toggle=”yes”>Saccharomyces cerevisiae</italic> Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins

Posted on Apr 4th, 2021 - By Lifang Yu and Mario Andrea Marchisio

[ASAP] An Automated Tabletop Continuous Culturing System with Multicolor Fluorescence Monitoring for Microbial Gene Expression and Long-Term Population Dynamics

[ASAP] Genetically Intact Bioengineered Spores of <italic toggle=”yes”>Bacillus</italic> <italic toggle=”yes”>subtilis</italic>

Posted on Mar 12th, 2021 - By Juan F. Quijano and Ozgur Sahin

[ASAP] Engineering Transcriptional Interference through RNA Polymerase Processivity Control

[ASAP] Heterologous Expression of Mersacidin in <italic toggle=”yes”>Escherichia coli</italic> Elucidates the Mode of Leader Processing

[ASAP] Genetically Engineered Methanotroph as a Platform for Bioaugmentation of Chemical Pesticide Contaminated Soil

[ASAP] CRISPRi-Mediated NIMPLY Logic Gate for Fine-Tuning the Whole-Cell Sensing toward Simple Urine Glucose Detection

Posted on Feb 9th, 2021 - By Shih-I Tan and I-Son Ng

[ASAP] Toolkit for Quickly Generating and Characterizing Molecular Probes Specific for SARS-CoV-2 Nucleocapsid as a Primer for Future Coronavirus Pandemic Preparedness

Posted on Feb 3rd, 2021 - By Laura Jo Sherwood and Andrew Hayhurst

[ASAP] Allosteric Regulation of DNA Circuits Enables Minimal and Rapid Biosensors of Small Molecules

Posted on Jan 22nd, 2021 - By Alan F. Rodríguez-Serrano and I-Ming Hsing

[ASAP] Optimization of a <italic toggle=”yes”>p</italic>-Coumaric Acid Biosensor System for Versatile Dynamic Performance

Posted on Dec 30th, 2020 - By Tian Jiang, Chenyi Li, and Yajun Yan

[ASAP] Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production

[ASAP] A Standardized Inverter Package Borne by Broad Host Range Plasmids for Genetic Circuit Design in Gram-Negative Bacteria

[ASAP] Correction to Constructing a Novel Biosynthetic Pathway for the Production of Glycolate from Glycerol in <italic toggle=”yes”>Escherichia coli</italic>

[ASAP] Engineered Bacterial Production of Volatile Methyl Salicylate

Posted on Dec 17th, 2020 - By Tiffany Chien, Drew R. Jones, and Tal Danino

[ASAP] Metabolic Engineering of <italic toggle=”yes”>Escherichia coli</italic> for <italic toggle=”yes”>De Novo</italic> Production of 1,5-Pentanediol from Glucose

[ASAP] A Logic NAND Gate for Controlling Gene Expression in a Circadian Rhythm in Cyanobacteria

Posted on Dec 2nd, 2020 - By Mieun Lee and Han Min Woo

[ASAP] Living Therapeutics: The Next Frontier of Precision Medicine

[ASAP] An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models

Posted on Oct 14th, 2020 - By Alexander C. Reis and Howard M. Salis

[ASAP] Isothermal SARS-CoV-2 Diagnostics: Tools for Enabling Distributed Pandemic Testing as a Means of Supporting Safe Reopenings

[ASAP] Reading and Writing Digital Information in TNA

[ASAP] Conditional Protein Rescue by Binding-Induced Protective Shielding

Posted on Oct 6th, 2020 - By Andrew S. Gaynor and Wilfred Chen

[ASAP] Reconstitution of the Ornithine Cycle with Arginine:Glycine Amidinotransferase to Engineer <italic toggle=”yes”>Escherichia coli</italic> into an Efficient Whole-Cell Catalyst of Guanidinoacetate

Posted on Aug 6th, 2020 - By pthomas2

[ASAP] Engineering <italic toggle=”yes”>Yarrowia lipolytica</italic> as a Chassis for <italic toggle=”yes”>De Novo</italic> Synthesis of Five Aromatic-Derived Natural Products and Chemicals

Posted on Jul 22nd, 2020 - By pthomas2

[ASAP] Programmed Allelic Mutagenesis of a DNA Polymerase with Single Amino Acid Resolution

Posted on Jun 29th, 2020 - By pthomas2

[ASAP] Artificial Protein-Responsive Riboswitches Upregulate Non-AUG Translation Initiation in Yeast

Posted on Jun 22nd, 2020 - By Fumihiro Horie, Kei Endo*, and Koichi Ito*

[ASAP] Harnessing Natural Modularity of Metabolism with Goal Attainment Optimization to Design a Modular Chassis Cell for Production of Diverse Chemicals

Posted on Jun 22nd, 2020 - By Sergio Garcia†‡ and Cong T. Trinh*†‡

[ASAP] CRISPRi-dCas12a: A dCas12a-Mediated CRISPR Interference for Repression of Multiple Genes and Metabolic Engineering in Cyanobacteria

Posted on May 18th, 2020 - By Sun Young Choi†‡ and Han Min Woo*†‡

[ASAP] Translation Related Factors Improve the Productivity of a <italic toggle=”yes”>Streptomyces</italic>-Based Cell-Free Protein Synthesis System

Posted on May 5th, 2020 - By Huiling Xu, Wan-Qiu Liu, and Jian Li*

[ASAP] The Protection Role of Magnesium Ions on Coupled Transcription and Translation in Lyophilized Cell-Free System

[ASAP] Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin

Posted on Mar 10th, 2020 - By pthomas2

[ASAP] MEMO: A Method for Computing Metabolic Modules for Cell-Free Production Systems

Posted on Mar 2nd, 2020 - By Axel von Kamp† and Steffen Klamt*†

[ASAP] A New Biosensor for Stilbenes and a Cannabinoid Enabled by Genome Mining of a Transcriptional Regulator

[ASAP] Development of a Quorum-Sensing Based Circuit for Control of Coculture Population Composition in a Naringenin Production System

[ASAP] Highly Efficient Biosynthesis of Hypoxanthine in <italic toggle=”yes”>Escherichia coli</italic> and Transcriptome-Based Analysis of the Purine Metabolism

[ASAP] Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in <italic toggle=”yes”>Saccharomyces cerevisiae</italic>

[ASAP] Engineering a DNAzyme-Based Operon System for the Production of DNA Nanoscaffolds in Living Bacteria

[ASAP] Reinforcement Learning for Bioretrosynthesis

Posted on Dec 30th, 2019 - By pthomas2

[ASAP] Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium <italic toggle=”yes”>Synechococcus</italic> sp. UTEX 2973

[ASAP] Orthogonal Protein-Responsive mRNA Switches for Mammalian Synthetic Biology

Posted on Dec 13th, 2019 - By pthomas2

[ASAP] Unravelling the Biosynthetic Flexibility of UK-2A Enables Enzymatic Synthesis of Its Structural Variants

Posted on Nov 25th, 2019 - By pthomas2

[ASAP] Enhanced Population Control in a Synthetic Bacterial Consortium by Interconnected Carbon Cross-Feeding

[ASAP] Interkingdom Genetic Mix-and-Match To Produce Novel Sunscreens

Posted on Nov 7th, 2019 - By Andrew R. Osborn and Taifo Mahmud*

[ASAP] <italic toggle=”yes”>In Vitro</italic> Transcriptional Regulation <italic toggle=”yes”>via</italic> Nucleic-Acid-Based Transcription Factors

[ASAP] Improving Chromosome Synthesis with a Semiquantitative Phenotypic Assay and Refined Assembly Strategy

Posted on Sep 19th, 2019 - By pthomas2

[ASAP] Low-Burden Biological Feedback Controllers for Near-Perfect Adaptation

Posted on Sep 12th, 2019 - By Harrison Steel* and Antonis Papachristodoulou*

[ASAP] Modular Thermal Control of Protein Dimerization

[ASAP] Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery

[ASAP] Development of a Vanillate Biosensor for the Vanillin Biosynthesis Pathway in <italic toggle=”yes”>E. coli</italic>

[ASAP] A Model for the Spatiotemporal Design of Gene Regulatory Circuits<xref rid=”notes-00″ ref-type=”statement”>†</xref>

[ASAP] Construction of Genetic Logic Gates Based on the T7 RNA Polymerase Expression System in <italic toggle=”yes”>Rhodococcus opacus</italic> PD630

Posted on Aug 6th, 2019 - By Drew M. DeLorenzo† and Tae Seok Moon*†‡

[ASAP] Genetic Selection for Small Molecule Production in Competitive Microfluidic Droplets

Posted on Aug 4th, 2019 - By pthomas2

[ASAP] Reversing an Extracellular Electron Transfer Pathway for Electrode-Driven Acetoin Reduction

[ASAP] Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution

[ASAP] Genetic Compatibility and Extensibility of Orthogonal Replication

Posted on May 21st, 2019 - By Alex A. Javanpour, Chang C. Liu

[ASAP] Elucidating the Determinants of Polymerase Specificity by Microfluidic-Based Deep Mutational Scanning

Posted on May 12th, 2019 - By Ali Nikoomanzar, Derek Vallejo, John C. Chaput

[ASAP] RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids

[ASAP] Synthetic Bistability and Differentiation in Yeast

Posted on Apr 30th, 2019 - By Yaoyu Yang, Jennifer L. Nemhauser, Eric Klavins

[ASAP] Combinatorial Sensor Design in <italic toggle=”yes”>Caulobacter crescentus</italic> for Selective Environmental Uranium Detection

Posted on Apr 2nd, 2019 - By Dan M. Park, Michael J. Taffet

[ASAP] Building an Inducible T7 RNA Polymerase/T7 Promoter Circuit in <italic toggle=”yes”>Synechocystis</italic> sp. PCC6803

Posted on Apr 2nd, 2019 - By Haojie Jin, Peter Lindblad, Devaki Bhaya

[ASAP] Systematic Evaluation of Genetic and Environmental Factors Affecting Performance of Translational Riboswitches

Posted on Apr 1st, 2019 - By R. Kent, N. Dixon

[ASAP] An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries

[ASAP] Modeling Genetic Circuit Behavior in Transiently Transfected Mammalian Cells

Posted on Mar 27th, 2019 - By Junmin Wang, Samuel A. Isaacson, Calin Belta

[ASAP] Enhanced Pyruvate Production in <italic toggle=”yes”>Candida glabrata</italic> by Engineering ATP Futile Cycle System

[ASAP] Structural Characterization of a Synthetic Tandem-Domain Bacterial Microcompartment Shell Protein Capable of Forming Icosahedral Shell Assemblies

[ASAP] Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium <italic toggle=”yes”>Anabaena</italic> sp. PCC 7120

Posted on Mar 18th, 2019 - By Akiyoshi Higo, Shigeki Ehira

[ASAP] Desymmetrization of Cyclodepsipeptides by Assembly Mode Switching of Iterative Nonribosomal Peptide Synthetases

[ASAP] Measuring Amber Initiator tRNA Orthogonality in a Genomically Recoded Organism

[ASAP] Temporal Pattern Recognition through Analog Molecular Computation

Posted on Mar 13th, 2019 - By Jackson O’Brien, Arvind Murugan

[ASAP] Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells

Posted on Mar 6th, 2019 - By Shaobin Guo, Richard M. Murray

[ASAP] Synthetic Inducible Regulatory Systems Optimized for the Modulation of Secondary Metabolite Production in <italic toggle=”yes”>Streptomyces</italic>

Posted on Mar 4th, 2019 - By Chang-Hun Ji, Hiyoung Kim, Hahk-Soo Kang

[ASAP] Metabolic Engineering of a Homoserine-Derived Non-Natural Pathway for the <italic toggle=”yes”>De Novo</italic> Production of 1,3-Propanediol from Glucose

[ASAP] Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration

Posted on Feb 22nd, 2019 - By Xenia Meshik, Patrick R. O’Neill, N. Gautam

[ASAP] Combining 26s rDNA and the Cre-loxP System for Iterative Gene Integration and Efficient Marker Curation in <italic toggle=”yes”>Yarrowia lipolytica</italic>

[ASAP] Redirecting Metabolic Flux <italic toggle=”yes”>via</italic> Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in <italic toggle=”yes”>Escherichia coli</italic>

[ASAP] Antisense RNA-Based Strategy for Enhancing Surfactin Production in <italic toggle=”yes”>Bacillus subtilis</italic> TS1726 via Overexpression of the Unconventional Biotin Carboxylase II To Enhance ACCase Activity

Posted on Jan 31st, 2019 - By Miaomiao Wang, Huimin Yu, Zhongyao Shen

[ASAP] Engineering a New Class of Anti-LacI Transcription Factors with Alternate DNA Recognition

Posted on Jan 20th, 2019 - By Ronald E. Rondon, Corey J. Wilson

[ASAP] Synthetic Transcription Factors Switch from Local to Long-Range Control during Cell Differentiation

Posted on Jan 17th, 2019 - By Takeo Wada, Sandrine Wallerich, Attila Becskei

[ASAP] An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism

Posted on Jan 16th, 2019 - By Sean Lund, Rachael Hall, Gavin J Williams

[ASAP] Metabolic Engineering of <italic toggle=”yes”>Saccharomyces cerevisiae</italic> for Production of Shinorine, a Sunscreen Material, from Xylose

[ASAP] Biological Engineered Living Materials: Growing Functional Materials with Genetically Programmable Properties

Posted on Jan 9th, 2019 - By Charlie Gilbert, Tom Ellis

[ASAP] Structure-Based Design of Versatile Biosensors for Small Molecules Based on the PAS Domain of a Thermophilic Histidine Kinase

Posted on Dec 10th, 2018 - By Kai U. Cormann, Meike Baumgart, Michael Bott

[ASAP] Retroelement-Based Genome Editing and Evolution

[ASAP] Transcription Driven by Reversible Photocontrol of Hyperstable G-Quadruplexes

Posted on Oct 16th, 2018 - By Shinzi Ogasawara

[ASAP] Enhancing Light-Driven 1,3-Propanediol Production by Using Natural Compartmentalization of Differentiated Cells

Posted on Sep 19th, 2018 - By Hongyu Liu, Jun Ni, Ping Xu, Fei Tao