Structures and function of the amino acid polymerase cyanophycin synthetase

August 12th, 2021 by Itai Sharon

Nature Chemical Biology, Published online: 12 August 2021; doi:10.1038/s41589-021-00854-y

Structures of three cyanophycin synthetases reveal how the constituent glutathione synthetase and muramyl ligase-like domains cooperate to make cyanophycin, a poly-aspartate chain with arginine residues attached to the sidechains by isopeptide bonds.
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Addendum: Cryptic phosphorylation in nucleoside natural product biosynthesis

August 11th, 2021 by Matthew M. Draelos

Nature Chemical Biology, Published online: 11 August 2021; doi:10.1038/s41589-021-00867-7

Addendum: Cryptic phosphorylation in nucleoside natural product biosynthesis
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Accelerated RNA detection using tandem CRISPR nucleases

August 5th, 2021 by Tina Y. Liu

Nature Chemical Biology, Published online: 05 August 2021; doi:10.1038/s41589-021-00842-2

Deploying two unrelated CRISPR nucleases in tandem, with multiplexed CRISPR RNAs and a chemically stabilized activator, creates a simple, one-step assay that can rapidly detect attomolar concentrations of RNA without needing target amplification.

A giant ubiquitin ligase

August 3rd, 2021 by Hidde Ploegh

Nature Chemical Biology, Published online: 03 August 2021; doi:10.1038/s41589-021-00860-0

The structure of a giant ubiquitin E3 ligase sheds light on its activation in a substrate-dependent manner and shows how a single E3 enzyme uses distinct recognition modules to confer substrate specificity.

A topological switch in CFTR modulates channel activity and sensitivity to unfolding

August 2nd, 2021 by Daniel Scholl

Nature Chemical Biology, Published online: 02 August 2021; doi:10.1038/s41589-021-00844-0

The cystic fibrosis transmembrane conductance regulator anion channel can adopt an alternate conformation of its nucleotide-binding domain, which affects channel activity and, under certain conditions, leads to unfolding and protein degradation.
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Designer membraneless organelles sequester native factors for control of cell behavior

August 2nd, 2021 by Mikael V. Garabedian

Nature Chemical Biology, Published online: 02 August 2021; doi:10.1038/s41589-021-00840-4

Controlled assembly of synthetic intracellular condensates from engineered disordered proteins enables control of cellular activities such as proliferation and division through recruitment, sequestration and insulation of endogenous client proteins.
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Insulating proteins drop by drop

August 2nd, 2021 by Ulrich Krauss

Nature Chemical Biology, Published online: 02 August 2021; doi:10.1038/s41589-021-00846-y

Liquid–liquid phase separation, yielding membraneless organelles, allows for the sequestration and functional insulation of cellular proteins. A modularly built, synthetic membraneless organelle platform enables efficient control over endogenous cellular activities by knockdown of protein function or controlled protein release.

Disorder is a critical component of lipoprotein sorting in Gram-negative bacteria

July 29th, 2021 by Jessica El Rayes

Nature Chemical Biology, Published online: 29 July 2021; doi:10.1038/s41589-021-00845-z

Approximately half of lipoproteins destined to the Escherichia coli outer membrane display intrinsically disordered linker peptides at their N termini, which are required for optimal trafficking by the Lol lipoprotein sorting system.
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[ASAP] HDAC6 Substrate Discovery Using Proteomics-Based Substrate Trapping: HDAC6 Deacetylates PRMT5 to Influence Methyltransferase Activity

July 26th, 2021 by Inosha D. Gomes, Udana V. Ariyaratne, and Mary Kay H. Pflum

TOC Graphic

ACS Chemical Biology
DOI: 10.1021/acschembio.1c00303
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[ASAP] <italic toggle=”yes”>In Vitro</italic> Reconstitution of the Pantothenic Acid Degradation Pathway in <italic toggle=”yes”>Ochrobactrum anthropi</italic>

July 26th, 2021 by Yuping Liu, Siting Pan, Xinshuai Zhang, and Hua Huang

TOC Graphic

ACS Chemical Biology
DOI: 10.1021/acschembio.1c00492
  • Posted in ACS Chemical Biology, Publications
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