A giant ubiquitin ligase

August 3rd, 2021 by Hidde Ploegh

Nature Chemical Biology, Published online: 03 August 2021; doi:10.1038/s41589-021-00860-0

The structure of a giant ubiquitin E3 ligase sheds light on its activation in a substrate-dependent manner and shows how a single E3 enzyme uses distinct recognition modules to confer substrate specificity.

A topological switch in CFTR modulates channel activity and sensitivity to unfolding

August 2nd, 2021 by Daniel Scholl

Nature Chemical Biology, Published online: 02 August 2021; doi:10.1038/s41589-021-00844-0

The cystic fibrosis transmembrane conductance regulator anion channel can adopt an alternate conformation of its nucleotide-binding domain, which affects channel activity and, under certain conditions, leads to unfolding and protein degradation.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on A topological switch in CFTR modulates channel activity and sensitivity to unfolding

Designer membraneless organelles sequester native factors for control of cell behavior

August 2nd, 2021 by Mikael V. Garabedian

Nature Chemical Biology, Published online: 02 August 2021; doi:10.1038/s41589-021-00840-4

Controlled assembly of synthetic intracellular condensates from engineered disordered proteins enables control of cellular activities such as proliferation and division through recruitment, sequestration and insulation of endogenous client proteins.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Designer membraneless organelles sequester native factors for control of cell behavior

Insulating proteins drop by drop

August 2nd, 2021 by Ulrich Krauss

Nature Chemical Biology, Published online: 02 August 2021; doi:10.1038/s41589-021-00846-y

Liquid–liquid phase separation, yielding membraneless organelles, allows for the sequestration and functional insulation of cellular proteins. A modularly built, synthetic membraneless organelle platform enables efficient control over endogenous cellular activities by knockdown of protein function or controlled protein release.

Disorder is a critical component of lipoprotein sorting in Gram-negative bacteria

July 29th, 2021 by Jessica El Rayes

Nature Chemical Biology, Published online: 29 July 2021; doi:10.1038/s41589-021-00845-z

Approximately half of lipoproteins destined to the Escherichia coli outer membrane display intrinsically disordered linker peptides at their N termini, which are required for optimal trafficking by the Lol lipoprotein sorting system.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Disorder is a critical component of lipoprotein sorting in Gram-negative bacteria

Metabolic engineering strategies to enable microbial utilization of C1 feedstocks

July 26th, 2021 by Wei Jiang

Nature Chemical Biology, Published online: 26 July 2021; doi:10.1038/s41589-021-00836-0

A survey of natural metabolic pathways for C1 substrates (methane, methanol, carbon dioxide, and carbon monoxide) highlights past efforts and recent progress, and informs future opportunities to create synthetic C1-utilizing microbes.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Metabolic engineering strategies to enable microbial utilization of C1 feedstocks

Prospecting for natural products by genome mining and microcrystal electron diffraction

July 26th, 2021 by Lee Joon Kim

Nature Chemical Biology, Published online: 26 July 2021; doi:10.1038/s41589-021-00834-2

Combined use of microcrystal electron diffraction and genome mining for biosynthetic gene clusters enables the rapid structural elucidation of natural products, including a newly discovered 2-pyridone compound and a revised structure of fischerin.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Prospecting for natural products by genome mining and microcrystal electron diffraction

Untying knots with force

July 26th, 2021 by Pan T. X. Li

Nature Chemical Biology, Published online: 26 July 2021; doi:10.1038/s41589-021-00856-w

RNA knot-like structures function as an efficient physical barrier to RNA exoribonucleases. Single-molecule mechanical manipulation is used to unfold these structures and unravel the cause of their unusual mechanical resistance from a different direction.

Central dogma, redefined

July 26th, 2021 by Yiyun Song

Nature Chemical Biology, Published online: 26 July 2021; doi:10.1038/s41589-021-00850-2

Central dogma, redefined

A molecular shape-shifter

July 26th, 2021 by Karin Kühnel

Nature Chemical Biology, Published online: 26 July 2021; doi:10.1038/s41589-021-00848-w

A molecular shape-shifter