A molecular trap inside microtubules probes luminal access by soluble proteins
May 3rd, 2021 by Yuta Nihongaki
Nature Chemical Biology, Published online: 03 May 2021; doi:10.1038/s41589-021-00791-w
A chemically inducible technique for trapping cytosolic proteins in microtubules (MTs) in cells reveals that soluble proteins can enter the MT lumen by diffusion, while proteins forming a complex with tubulins can be incorporated at MT plus ends.A split CRISPR–Cpf1 platform for inducible genome editing and gene activation
August 12th, 2019 by Yuta Nihongaki
Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0338-y
Split Cpf1 pairs are identified to enable chemical- and light-induced genome editing via dimerization. Another pair of split Cpf1 can be used to activate gene expression with high efficiency in cells and in mice.