A molecular trap inside microtubules probes luminal access by soluble proteins

May 3rd, 2021 by Yuta Nihongaki

Nature Chemical Biology, Published online: 03 May 2021; doi:10.1038/s41589-021-00791-w

A chemically inducible technique for trapping cytosolic proteins in microtubules (MTs) in cells reveals that soluble proteins can enter the MT lumen by diffusion, while proteins forming a complex with tubulins can be incorporated at MT plus ends.
  • Posted in Nat Chem Biol, Publications
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A split CRISPR–Cpf1 platform for inducible genome editing and gene activation

August 12th, 2019 by Yuta Nihongaki

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0338-y

Split Cpf1 pairs are identified to enable chemical- and light-induced genome editing via dimerization. Another pair of split Cpf1 can be used to activate gene expression with high efficiency in cells and in mice.
  • Posted in Nat Chem Biol, Publications
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