Global Ablation of Mouse Rab11a Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion [Cell Biology]

September 30th, 2014 by Yu, S., Yehia, G., Wang, J., Stypulkowski, E., Sakamori, R., Jiang, P., Hernandez-Enriquez, E. B., Tran, T. S., Bonder, E. M., Guo, W., Gao, N.

Rab11a has been conceived as a prominent regulatory component of the recycling endosome, which acts as a nexus in the endo- and exo-cytotic networks. The precise in vivo role of Rab11a in mouse embryonic development is unknown. We globally ablated Rab11a and examined the phenotypic and molecular outcomes in Rab11a-null blastocysts and mouse embryonic fibroblasts (MEFs). Using multiple trafficking assays and complementation analyses, we determined, among multiple important membrane-associated and soluble cargos, the critical contribution of Rab11a vesicular traffic to the secretion of multiple soluble MMPs. Rab11a-null embryos were able to properly form normal blastocysts but died at peri-implantation stages. Our data suggested that Rab11a critically controls mouse blastocyst development and soluble MMP secretion.