Accelerated RNA detection using tandem CRISPR nucleases

August 5th, 2021 by Tina Y. Liu

Nature Chemical Biology, Published online: 05 August 2021; doi:10.1038/s41589-021-00842-2

Deploying two unrelated CRISPR nucleases in tandem, with multiplexed CRISPR RNAs and a chemically stabilized activator, creates a simple, one-step assay that can rapidly detect attomolar concentrations of RNA without needing target amplification.