Monobody-mediated alteration of enzyme specificity

August 31st, 2015 by Shun-ichi Tanaka

Nature Chemical Biology 11, 762 (2015). doi:10.1038/nchembio.1896

Authors: Shun-ichi Tanaka, Tetsuya Takahashi, Akiko Koide, Satoru Ishihara, Satoshi Koikeda & Shohei Koide

Current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. Here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzyme. Using the example of a β-galactosidase from Bacillus circulans, we efficiently identified monobodies that restricted its substrates for its transgalactosylation reaction and selectively enhanced the production of small oligosaccharide prebiotics.