A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system
July 3rd, 2017 by Paul H Opgenorth
Nature Chemical Biology 13, 938 (2017). doi:10.1038/nchembio.2418
Authors: Paul H Opgenorth, Tyler P Korman, Liviu Iancu & James U Bowie
A synthetic biochemistry module for production of bio-based chemicals from glucose
April 11th, 2016 by Paul H Opgenorth
Nature Chemical Biology 12, 393 (2016). doi:10.1038/nchembio.2062
Authors: Paul H Opgenorth, Tyler P Korman & James U Bowie
Synthetic biochemistry, the cell-free production of biologically based chemicals, is a potentially high-yield, flexible alternative to in vivo metabolic engineering. To limit costs, cell-free systems must be designed to operate continuously with minimal addition of feedstock chemicals. We describe a robust, efficient synthetic glucose breakdown pathway and implement it for the production of bioplastic. The system's performance suggests that synthetic biochemistry has the potential to become a viable industrial alternative.