Site-specific incorporation of phosphotyrosine using an expanded genetic code
June 12th, 2017 by Christian Hoppmann
Nature Chemical Biology 13, 842 (2017). doi:10.1038/nchembio.2406
Authors: Christian Hoppmann, Allison Wong, Bing Yang, Shuwei Li, Tony Hunter, Kevan M Shokat & Lei Wang
Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination.