SH2B1 and IRSp53 Promotes the Formation of Dendrites and Dendritic Branches [Neurobiology]

January 13th, 2015 by Chen, C.-J., Shih, C.-H., Chang, Y.-J., Hong, S.-J., Li, T.-N., Wang, L. H.-C., Chen, L.

SH2B1 is an adapter protein known to enhance neurite outgrowth. In this study, we provide evidence suggesting that SH2B1 level is increased during in vitro culture of hippocampal neurons and the β isoform (SH2B1β) is the predominant isoform. The fact that formation of filopodia is prerequisite for neurite initiation suggests that SH2B1 may regulate filopodium formation and thus neurite initiation. To investigate whether SH2B1 may regulate filopodium formation, the effect of SH2B1 and a membrane and actin regulator, IRSp53 (insulin receptor tyrosine kinase substrate p53), is investigated. Overexpressing both SH2B1β and IRSp53 significantly enhances filopodium formation, neurite outgrowth and branching. Both in vivo and in vitro data show that SH2B1 interacts with IRSp53 in hippocampal neurons. This interaction depends on the N-terminal proline-rich domains of SH2B1. In addition, SH2B1 and IRSp53 co-localize at the plasma membrane and their levels increase in the Triton X-100-insoluble fraction of developing neurons. These findings suggest that SH2B1-IRSp53 complexes promote the formation of filopoda, neurite initiation and neuronal branching.