Orthogonal fluorescent chemogenetic reporters for multicolor imaging

August 10th, 2020 by Alison G. Tebo

Nature Chemical Biology, Published online: 10 August 2020; doi:10.1038/s41589-020-0611-0

The fluorescent chemogenetic reporters greenFAST and redFAST were engineered by protein engineering. They display orthogonal fluorogen recognition and spectral properties allowing efficient multicolor imaging of proteins in live cells and organisms.